Cookies on CAB eBooks

Like most websites we use cookies. This is to ensure that we give you the best experience possible.

 

Continuing to use www.cabi.org  means you agree to our use of cookies. If you would like to, you can learn more about the cookies we use.

CAB eBooks

Ebooks on agriculture and the applied life sciences from CAB International

CABI Book Chapter

Cold hardiness in plants: molecular genetics, cell biology and physiology. Seventh International Plant Cold Hardiness Seminar, Sapporo, Japan, 10-15 July 2004.

Book cover for Cold hardiness in plants: molecular genetics, cell biology and physiology. Seventh International Plant Cold Hardiness Seminar, Sapporo, Japan, 10-15 July 2004.

Description

This book contains 16 papers presenting the latest research findings on plant freezing and chilling stress from major laboratories around the world. They focus on various aspects of molecular genetics and, in many cases, the use of transgenic plants to further our understanding of plant cold hardiness at the molecular level. Other papers include: vernalization genes in winter cereals; global analy...

Metrics

Chapter 12 (Page no: 167)

The role of the CBF-dependent signalling pathway in woody perennials.

A study was conducted to investigate the changes in phenotype and transcript profile of transgenic Populus constitutively expressing CBF1 from Arabidopsis thaliana (AtCBF1). Ectopic expression of AtCBF1 was sufficient to significantly increase the freezing tolerance (FT) of non-acclimatized leaves (-3.9°C for wild-type (WT) vs. -6.7°C and -7.1°C for transgenic lines 1 and 2, respectively) and stems (-4.1°C for WT vs. -5.2°C and -5.7°C for transgenic lines 1 and 2, respectively), comparable to WT plants after one week of cold acclimatization. Experiments with cDNA microarrays identified genes upregulated by ectopic AtCBF1 expression in Populus and demonstrated the strong conservation of the CBF regulon between Populus and Arabidopsis thaliana. Upon studying the induction kinetics and tissue specificity for four CBF paralogues identified from the Populus trichocarpa genome sequence (PtCBFs), results showed that all four PtCBFs are cold inducible in leaves, but only PtCBF1 and PtCBF3 show significant induction in stems, suggesting that the central role played by the CBF family of transcriptional activators in cold acclimatization of Arabidopsis thaliana has been maintained in Populus in the 50 million years since their divergence. Studies of the CBF regulon in a perennially dormant woody plant species like Populus strengthen the conclusion that the CBF low-temperature signalling pathway is not only conserved, but similarly seems to work through ABA and GA signalling elements in woody dicots.

Other chapters from this book

Chapter: 1 (Page no: 1) Global analysis of gene networks to solve complex abiotic stress responses. Author(s): Shinozaki, K. Yamaguchi-Shinozaki, K.
Chapter: 2 (Page no: 11) The CBF cold response pathways of Arabidopsis and tomato. Author(s): Vogel, J. T. Cook, D. Fowler, S. G. Thomashow, M. F.
Chapter: 3 (Page no: 30) Barley contains a large CBF gene family associated with quantitative cold-tolerance traits. Author(s): Skinner, J. S. Zitzewitz, J. von Marquez-Cedillo, L. Filichkin, T. Szűcs, P. Amundsen, K. Stockinger, E. J. Thomashow, M. F. Chen, T. H. H. Hayes, P. M.
Chapter: 4 (Page no: 53) Structural organization of barley CBF genes coincident with a QTL for cold hardiness. Author(s): Stockinger, E. J. Cheng, H. Skinner, J. S.
Chapter: 5 (Page no: 64) The genetic basis of vernalization responses in barley. Author(s): Cooper, L. L. D. Zitzewitz, J. von Skinner, J. S. Szűcs, P. Karsai, I. Francia, E. Stanca, A. M. Pecchioni, N. Laurie, D. A. Chen, T. H. H. Hayes, P. M.
Chapter: 6 (Page no: 76) Vernalization genes in winter cereals. Author(s): Kane, N. A. Danyluk, J. Sarhan, F.
Chapter: 7 (Page no: 88) A bulk segregant approach to identify genetic polymorphisms associated with cold tolerance in lucerne. Author(s): Castonguay, Y. Cloutier, J. Laberge, S. Bertrand, A. Michaud, R.
Chapter: 8 (Page no: 103) Ectopic overexpression of AtCBF1 in potato enhances freezing tolerance. Author(s): Pino, M. T. Skinner, J. S. Jeknić, Z. Park, E. J. Hayes, P. M. Chen, T. H. H.
Chapter: 9 (Page no: 124) Overexpression of a heat-inducible apx gene confers chilling tolerance to rice plants. Author(s): Sato, Y. Saruyama, H.
Chapter: 10 (Page no: 138) Physiological and morphological alterations associated with development of freezing tolerance in the moss Physcomitrella patens. Author(s): Minami, A. Nagao, M. Arakawa, K. Fujikawa, S. Takezawa, D.
Chapter: 11 (Page no: 153) Control of growth and cold acclimation in silver birch. Author(s): Aalto, M. K. Palva, E. T.
Chapter: 13 (Page no: 181) Functional role of winter-accumulating proteins from mulberry tree in adaptation to winter-induced stresses. Author(s): Fujikawa, S. Ukaji, N. Nagao, M. Yamane, K. Takezawa, D. Arakawa, K.
Chapter: 14 (Page no: 203) The role of compatible solutes in plant freezing tolerance: a case study on raffinose. Author(s): Hincha, D. K. Zuther, E. Hundertmark, M. Heyer, A. G.
Chapter: 15 (Page no: 219) Dehydration in model membranes and protoplasts: contrasting effects at low, intermediate and high hydrations. Author(s): Koster, K. L. Bryant, G.
Chapter: 16 (Page no: 235) Effect of plasma membrane-associated proteins on acquisition of freezing tolerance in Arabidopsis thaliana. Author(s): Tominaga, Y. Nakagawara, C. Kawamura, Y. Uemura, M.