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CAB eBooks

Ebooks on agriculture and the applied life sciences from CAB International

CABI Book Chapter

Biotechnology of major cereals.

Book cover for Biotechnology of major cereals.


This book contains 16 chapters focusing on the recombinant DNA techniques and transformation methods used by the commercial maize biotechnology sector, current traits and future trends of the global adoption of transgenic maize, specific breeding challenges and the current trends in applied sorghum biotechnology research, genetic modification methods, target traits and other relevant issues for ba...


Chapter 2 (Page no: 5)

Genetic transformation of maize: conventional methods and precision genome modification.

This chapter describes relevant technologies and their development in maize transformation for both random and targeted transgene integration. It focuses on the recombinant DNA techniques and transformation methods used by the commercial maize biotech sector. Although there has been considerable progress in improving transformation and event characterization technology, there are limitations to further efficiency gains from increasing transformation efficiency and higher throughput methodologies. The advantage of targeted integration is to generate only a small number of events in a locus of choice, as compared to screening a large number of randomly integrated events for stable and optimal transgene expression levels. Significant advancement has been made in this area, including the discovery of endonucleases to create DNA double-strand breaks, development of different strategies for HR (homologous recombination)- and NHEJ (non-homologous end-joining)-mediated recombination, which may occur at endogenous genome loci, as well as pre-integrated transgenic loci, for gene or trait stacking.

Other chapters from this book

Chapter: 1 (Page no: 1) Introduction: biotechnology of major cereals. Author(s): Jones, H. D.
Chapter: 3 (Page no: 18) Biotech maize: industry development and impact. Author(s): Coram, T. Bing, J. Storer, N.
Chapter: 4 (Page no: 48) Sorghum genetic transformation: current status and future target traits. Author(s): Anami, S. E. Zhang YuMiao Zhang LiXin Zhu Li Jing HaiChun
Chapter: 5 (Page no: 80) Barley as a cereal model for biotechnology applications. Author(s): Harwood, W.
Chapter: 6 (Page no: 88) Wheat biotechnology: recent developments and future trends. Author(s): Sparks, C. A. Jones, H. D.
Chapter: 7 (Page no: 104) The long and winding road of rice genetic modification technology and its potential. Author(s): Slamet-Loedin, I. H. Macovei, A.
Chapter: 8 (Page no: 119) Setaria viridis: a model for C4 crop biotechnology. Author(s): Eck, J. van Swartwood, K. Pidgeon, K.
Chapter: 9 (Page no: 125) Genome editing in cereals: current status and future potential. Author(s): Jones, H. D.
Chapter: 10 (Page no: 133) Anther culture for doubled haploids. Author(s): Karaoğlu, C. Salantur, A.
Chapter: 11 (Page no: 141) Chloroplast transformation in cereals. Author(s): Samnakay, P.
Chapter: 12 (Page no: 151) Cross-species silencing: plant-mediated RNAi for insect control. Author(s): Yu XiuDao Jones, H. D. Sun YongWei Wang GenPing Xia LanQin
Chapter: 13 (Page no: 165) Acrylamide in cereals: the problem, and potential genetic and agronomic solutions. Author(s): Halford, N. G. Curtis, T. Y.
Chapter: 14 (Page no: 179) Engineering cereal endosperm. Author(s): Opsahl-Sorteberg, H. G.
Chapter: 15 (Page no: 194) Key molecular and metabolic processes used for genetic engineering to improve freezing tolerance in cereals. Author(s): Soltész, A. Harwood, W. Kalapos, B. Vágújfalvi, A. Galiba, G.
Chapter: 16 (Page no: 206) Mergers and acquisitions in global ag-biotech. Author(s): Dunwell, J. M.

Chapter details

  • Author Affiliation
  • Dow AgroSciences, LLC, 9330 Zionsville Road, Indianapolis, IN 46268, USA.
  • Year of Publication
  • 2016
  • ISBN
  • 9781780645193
  • Record Number
  • 20163314711