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CABI Book Chapter

Mutation breeding in oil palm: a manual.

Book cover for Mutation breeding in oil palm: a manual.

Description

This manual provides an introduction to mutation breeding and practical protocols on mutation induction and mutant selection in oil palm (Elaeis guineensis). Topics covered include the brief history of plant mutation breeding; health and safety considerations and guidelines in mutation induction; radio-sensitivity testing; challenges, opportunities and options for mutation breeding in oil palm; pr...

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Chapter 2 (Page no: 15)

Health and safety considerations and guidelines.

Mutation induction is the most hazardous part of mutation breeding. The application of physical or chemical mutagens as well as the general handling of mutant materials in mutation breeding has associated high health and safety risks. Poor practice can be hazardous, resulting in minor or serious personal injuries, and even fatal accidents. Therefore, the use of standard health and safety protocols as guides in mutation induction activities is an absolute necessity. It is important to abide by the rules and regulations of mutation induction in order to avoid, or reduce to the barest minimum, injuries and accidents, as well as associated financial costs. With respect to chemical mutagens, these need specialist waste disposal systems after use. It is essential that mutation induction is carried out by specially trained personnel, and it is recommended that this is done in specialized institutes, or at least laboratories. International, regional and national service facilities are available (Chapter 6 of this manual). Certain rules and regulations constituting the operation guidelines may be generally applicable; however, depending on local requirements, some rules and regulations may be specific to a particular region or country. In certain jurisdictions, negligence in the strict adherence to health and safety protocols may incur sanctions such as fines, jail terms, or an embargo on field and laboratory research or commercial operations. This chapter highlights the standard operating protocols on health and safety issues relating to mutation induction for breeding in oil palm. Once mutation treatment has been carried out, subsequent steps (mutation detection, mutant line development and breeding) are relatively safe, but adherence to good laboratory, glasshouse and field practices is essential.

Other chapters from this book

Chapter: 1 (Page no: 3) Introduction. Author(s): Nur, F. Forster, B. P. Osei, S. A. Amiteye, S. Ciomas, J. Hoeman, S. Jankuloski, L.
Chapter: 3 (Page no: 23) Radio-sensitivity testing. Author(s): Nur, F. Forster, B. P. Osei, S. A. Amiteye, S. Ciomas, J. Hoeman, S. Jankuloski, L.
Chapter: 4 (Page no: 33) Options for mutation breeding in oil palm. Author(s): Nur, F. Forster, B. P. Osei, S. A. Amiteye, S. Ciomas, J. Hoeman, S. Jankuloski, L.
Chapter: 5 (Page no: 41) Protocol for developing mutant generations for mutant selection. Author(s): Nur, F. Forster, B. P. Osei, S. A. Amiteye, S. Ciomas, J. Hoeman, S. Jankuloski, L.
Chapter: 6 (Page no: 53) Services in irradiation treatments. Author(s): Nur, F. Forster, B. P. Osei, S. A. Amiteye, S. Ciomas, J. Hoeman, S. Jankuloski, L.