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CABI Book Chapter

Mutation breeding in oil palm: a manual.

Book cover for Mutation breeding in oil palm: a manual.

Description

This manual provides an introduction to mutation breeding and practical protocols on mutation induction and mutant selection in oil palm (Elaeis guineensis). Topics covered include the brief history of plant mutation breeding; health and safety considerations and guidelines in mutation induction; radio-sensitivity testing; challenges, opportunities and options for mutation breeding in oil palm; pr...

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Chapter 4 (Page no: 33)

Options for mutation breeding in oil palm.

Theoretical and practical options for mutation breeding in oil palm are discussed. In theory, haploids are considered the ideal targets, as the induced mutation can be fixed instantly on conversion to doubled haploids. However, haploid/doubled haploid technology is in its infancy in oil palm and therefore other practical targets need to be considered. The two obvious targets are pollen and seed, as these are produced in large numbers in breeding and commercial seed production. Schemes for pollen and seed irradiation and subsequent mutant population development are compared. The irradiation of germinated seed is currently considered to be the better approach in terms of convenience and time. A major constraint in mutation breeding in oil palm is the long life cycle. Oil palm has a long juvenile stage, and it takes 4-5 years from sowing a seed to getting seed of the next generation. Traditionally, mutant selection has relied on phenotypic selection, which can only take place in the second mutant generation (M2) due to the presence of physiological disorders and chimeras in the M1. However, now that the oil palm genome has been sequenced, it is feasible to select for mutants genotypically in the M1. Early detection of mutants is extremely valuable in oil palm, as it saves space in growing up only selected mutants in the field. Mutation breeding is a non-GM technique that has been used for over nine decades. New methods in genetic manipulation include gene editing, which has huge potential for the future but is currently still under debate as to whether it is considered a GM method and is subject to restrictive regulations.

Other chapters from this book

Chapter: 1 (Page no: 3) Introduction. Author(s): Nur, F. Forster, B. P. Osei, S. A. Amiteye, S. Ciomas, J. Hoeman, S. Jankuloski, L.
Chapter: 2 (Page no: 15) Health and safety considerations and guidelines. Author(s): Nur, F. Forster, B. P. Osei, S. A. Amiteye, S. Ciomas, J. Hoeman, S. Jankuloski, L.
Chapter: 3 (Page no: 23) Radio-sensitivity testing. Author(s): Nur, F. Forster, B. P. Osei, S. A. Amiteye, S. Ciomas, J. Hoeman, S. Jankuloski, L.
Chapter: 5 (Page no: 41) Protocol for developing mutant generations for mutant selection. Author(s): Nur, F. Forster, B. P. Osei, S. A. Amiteye, S. Ciomas, J. Hoeman, S. Jankuloski, L.
Chapter: 6 (Page no: 53) Services in irradiation treatments. Author(s): Nur, F. Forster, B. P. Osei, S. A. Amiteye, S. Ciomas, J. Hoeman, S. Jankuloski, L.