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Field trials in oil palm breeding: a manual.

Book cover for Field trials in oil palm breeding: a manual.

Description

A central objective in Verdant's mission is to develop better, more productive and more sustainable cultivars of oil palm (Elaeis guineensis), rubber and other plantation crops, particularly through plant breeding. Field testing is an essential component in selecting and developing new varieties with superior performance for yield, quality, pest and disease resistance and in meeting new market dem...

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Chapter 9 (Page no: 85)

Pre-trial screening using DNA diagnostics.

DNA analysis provides an opportunity to screen for certain traits prior to field planting. This is particularly important in screening for shell thickness. The shell thickness gene (Sh) is probably the most valuable gene in oil palm breeding and commerce. Pre-field planting screening is important as selection for dura and tenera types can be made and (sterile) pisiferas may be eliminated, thus saving on time and land space. This also allows for more robust field trialling as dura and tenera types can be separated. Nursery seedlings offer a convenient phase in which to sample leaves, extract and analyse DNA, thus selections can be made before field planting. In addition to the Sh gene, screening for other important traits is now possible (e.g. virescens and mantled fruit). This chapter gives an overview of pre-trial screening using currently available DNA diagnostics, but this area of work is developing quickly and set to expand in the future. Practical details of the DNA protocols involved are not given as these are in another manual in this series (Anwar et al., 2019). DNA analyses can be carried out in-house or outsourced to DNA service laboratories.

Other chapters from this book

Chapter: 1 (Page no: 3) Introduction. Author(s): Sitepu, B. Laksono, N. D. Setiawati, U. Nur, F. Rahmaningsih, M. Anwar, Y. Widodo, P. Forster, B. P. Purba, A. R.
Chapter: 2 (Page no: 15) Health and safety considerations. Author(s): Sitepu, B. Laksono, N. D. Setiawati, U. Nur, F. Rahmaningsih, M. Anwar, Y. Widodo, P. Forster, B. P. Purba, A. R.
Chapter: 3 (Page no: 23) Pre-trialling considerations and activities. Author(s): Sitepu, B. Laksono, N. D. Setiawati, U. Nur, F. Rahmaningsih, M. Anwar, Y. Widodo, P. Forster, B. P. Purba, A. R.
Chapter: 4 (Page no: 33) Land preparation. Author(s): Sitepu, B. Laksono, N. D. Setiawati, U. Nur, F. Rahmaningsih, M. Anwar, Y. Widodo, P. Forster, B. P. Purba, A. R.
Chapter: 5 (Page no: 45) Material preparation. Author(s): Sitepu, B. Laksono, N. D. Setiawati, U. Nur, F. Rahmaningsih, M. Anwar, Y. Widodo, P. Forster, B. P. Purba, A. R.
Chapter: 6 (Page no: 49) Trial planting. Author(s): Sitepu, B. Laksono, N. D. Setiawati, U. Nur, F. Rahmaningsih, M. Anwar, Y. Widodo, P. Forster, B. P. Purba, A. R.
Chapter: 7 (Page no: 59) Recording. Author(s): Sitepu, B. Laksono, N. D. Setiawati, U. Nur, F. Rahmaningsih, M. Anwar, Y. Widodo, P. Forster, B. P. Purba, A. R.
Chapter: 8 (Page no: 77) Ganoderma trials. Author(s): Sitepu, B. Laksono, N. D. Setiawati, U. Nur, F. Rahmaningsih, M. Anwar, Y. Widodo, P. Forster, B. P. Purba, A. R.